How do I perform a 2, 3, 4, 5 or 10 times dilution?

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How do I perform a 2, 3, 4, 5 or 10 times dilution?

Decide on the degree of sample dilution required
Fill dilution tube with sample to one of the sample marks as appropriate (example: if a 5 times dilution is required, fill to the x5 mark)
Top the tube up with de-ionised water up to the line marked ‘Deionised water
Cap the tube and mix the solution
Use the diluted sample and carry out the test as normal
Multiply the test result by the dilution factor used (example: if the tube was originally filled to the x5 mark, then the test kit result should be multiplied by 5 to give the concentration in the original sample)

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