What is the membrane filtration technique?

The membrane filtration (MF) method can be used to reliably test large numbers of water sample, and is US EPA approved for measuring coliforms.

The water sample is filtered through the membrane filter using a vacuum to pull the water through the membrane. The membrane filter is then placed onto a nutrient source gel in a petri dish and incubated for 24 h at 37 °C. The Coliform and non-Coliform colonies can then be counted. Often pH indicators are used to distinguish between different colony types.

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