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The designated bathing water sample point is usually identified by signage for each location and a sample can be taken anywhere along the transept. For SirenBW the designated bathing water sample point can continue to be used or other higher risk sample locations can be included to understand more intensely the nature of risk to bathing water users. Samples should be taken at least 30cm below the surface of the water and avoid capture of solid particles where possible.
The SirenBW method evaluates a parameter called Endotoxin Risk (ER), a general measurement of the likely health impact of bathing water quality. Endotoxin Risk includes faecal pollution through to toxic Blue Green Algae (BGA) and covers a wide range of pathogens. The Siren method will detect the changing profile of the bathing water with respect to the likely impact on user health by reporting elevated levels of ER.
The science behind the Siren method is a patented variation of the classic endotoxin assay used to validate the safety of pharmaceuticals and other products where safety is critical. The method detects the presence of lipopolysaccharide (LPS), the underlying cause of an inflammatory or immune response to the presence of toxic substances or pathogens. Palintest has integrated the core science into a field-ready colorimetric assay for bathing water quality risk screening within the SirenBW kit.
E.coli is one of the two parameters used to define the quality of a bathing water due to its ability to indicate the likely presence of faecal contamination. The Siren method is calibrated using an E. coli standard and there is a linear relationship between E. coli and Endotoxin Risk. It is important to remember however that the Siren assay will detect additional pathogens as well as E. coli.
The presence of Intestinal Enterococci is the second of two parameters used to classify bathing water quality according to the European Union Bathing Water Directive, the first being E. coli. The direct relationship between Intestinal Enterococci and the Endotoxin Risk reported by the Siren method is a weak one due to the small concentration of the active lipopolysaccharide (LPS) sites available for detection. However, a situation where high levels of Intestinal Enterococci and low levels of LPS are present is extremely unlikely to occur.
24th May 2019
Our team have compiled a list of their top tips to help ensure you get the best out of your Palintest equipment.
24th May 2019
In this short support document, we explain the difference between accuracy and precision.